The Dorothy Perkins and Burton store on Lincoln High Street is set to close its doors for good from April 22, staff have told Lincolnshire Business.
More than 20 staff could be made redundant if they are not able to be relocated within the company.
Both Dorothy Perkins and Burton are part of the Arcadia Group, which formerly owned BHS.
The group recently closed around 160 BHS stores across the county, including two stores in Lincoln.
Arcadia Group has not responded when contacted by Lincolnshire Business in regards to the news.